Effect of Adlay Seed Extract on the Level of Neuroprotection Gene Expression in Human Nasal Orbital Mesenchymal Stem Cells
Hamidreza Hassanipour 1 *, Ali Azimi1 , Fatemeh Sanie-jahromi 1 , Pardis Bostanian 1 , Behzad Khademi 1
- Poostchi Ophthalmology Research Center, Department of Ophthalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: This study aimed to explore how adlay seed extract impacts the gene expression of key components within the neuroprotection pathway (NGF, TRKB, MAPK, PI3K) in human nasal orbital mesenchymal stem cells (OAMSCs).
Methods: Nasal OAMSCs, with a density of 106 cells/ 10 cm2, were subjected to a 24-hour exposure to adlay seed extracts (namely the methanolic (MeOH) and residual (Res) fractions at a concentration of 1 mg/ml). The control group got an identical solution without the extract at the same time and under the same circumstances. We next measured the relative expression levels of NGF, TRKB, MAPK, and PI3K in the treated cells in comparison to the control group using real-time PCR.
Results: Our data revealed that both the MeOH and Res extracts of adlay seed led to a noteworthy upregulation of NGF in nasal OAMSCs. MeOH extract also led to the overexpression of TRKB in OAMSCs, while the other genes understudy, were not altered.
Conclusion: Our study marks the initial documentation of the stimulating impact of adlay extract on the transcriptional level of neurotrophin NGF and the TRKB receptor in nasal OAMSCs. We also showed that the extraction method could significantly affect the molecular-level properties of adlay. This preliminary study has the potential to lay the foundation for future advancements in the treatment of retinal neurodegenerative disorders and ocular regenerative medicine.
